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Addgene

pcDNA/TO/EGFPmiR21
(Plasmid #10827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5038
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pcDNA/TO/EGFPmiR21
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    778

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA/TO/EGFPmiR21 was a gift from Thomas Tuschl (Addgene plasmid # 10827 ; http://n2t.net/addgene:10827 ; RRID:Addgene_10827)

  • For your References section:

    Sequence-specific inhibition of microRNA- and siRNA-induced RNA silencing. Meister G, Landthaler M, Dorsett Y, Tuschl T. RNA. 2004 Mar . 10(3):544-50. 10.1261/rna.5235104 PubMed 14970398
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