sAJM.1505
(Bacterial strain #108253)

• Purpose: Marionette-Pro. Marionette cluster inserted in the direction of leading strand replication, between 3,720,027 and 3,721,644 in E. coli BL21
• Depositing Lab: Christopher Voigt
• Publication: Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3.

Backbone

• Vector backbone: N/A

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 5 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): This is a strain
• Growth instructions: Recommended to grow without antibiotics
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • Marionette insert
  • Verification Protocol for Marionette Strains

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The three new “Marionette” strains contain the Marionette cluster, which expresses 12 inducible transcription factors, from the genome. Plasmids pAJM.711, pAJM.712, pAJM.713, pAJM.714, pAJM.715, pAJM.717, pAJM.716, pAJM.718, pAJM.719, pAJM.1459, pAJM.721 are reporters for those 12 transcription factors. For examples: TetR is being expressed from the genome of the Marionette strains, and pAJM.717 has a promoter that is repressed by TetR (PTet*) driving YFP. pAJM.717 should not produce YFP, until the cell is exposed to anhydrotetracycline (aTc). Other plasmids from our laboratory encode both the reporter and the transcription factor, and should be used in a regular (non-Marionette) strain. For example: pAJM.011 expresses TetR and contains a PTet*-YFP reporter module.

Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.