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Addgene

pgRNA-CGG
(Plasmid #108248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pgRNA-modified
  • Backbone manufacturer
    Addgene 84477
  • Total vector size (bp) 8319
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting GGCGGCGGCGGCGGCGGCGG
  • gRNA/shRNA sequence
    none
  • Species
    Synthetic
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AarI (destroyed during cloning)
  • 3′ cloning site AarI (destroyed during cloning)
  • 5′ sequencing primer gaaactcaccctaactg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contain a CMV-Puro-T2A-mCherry cassette allowing for FACS isolation or puromycin selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pgRNA-CGG was a gift from Rudolf Jaenisch (Addgene plasmid # 108248 ; http://n2t.net/addgene:108248 ; RRID:Addgene_108248)

  • For your References section:

    Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R. Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012. 10.1016/j.cell.2018.01.012 PubMed 29456084
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