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Fuw-AcrIIA4-P2A-GFP
(Plasmid #108247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Fuw
  • Backbone size w/o insert (bp) 10043
  • Total vector size (bp) 10331
  • Modifications to backbone
    Fuw-HA-P2A-EGFP
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AcrIIA4-NLS-HA
  • Species
    Synthetic
  • Insert Size (bp)
    288
  • Entrez Gene
    NEWENTRY
  • Promoter Ubc
  • Tag / Fusion Protein
    • HA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer aactatgcgctcggg
  • 3′ sequencing primer taaagcagcgtatcc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The sequence of AcrIIA4 was reported by Joseph Bondy-Denomy's laboratory "Inhibition of CRISPR-Cas9 with Bacteriophage Proteins", Cell, 2016.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Fuw-AcrIIA4-P2A-GFP was a gift from Rudolf Jaenisch (Addgene plasmid # 108247 ; http://n2t.net/addgene:108247 ; RRID:Addgene_108247)

  • For your References section:

    Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Liu XS, Wu H, Krzisch M, Wu X, Graef J, Muffat J, Hnisz D, Li CH, Yuan B, Xu C, Li Y, Vershkov D, Cacace A, Young RA, Jaenisch R. Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012. 10.1016/j.cell.2018.01.012 PubMed 29456084
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