Fuw-dCas9-Tet1CD-P2A-BFP
(Plasmid #108245)

• Purpose: Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP
• Depositing Lab: Rudolf Jaenisch
• Publication: Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)30049-7. doi: 10.1016/j.cell.2018.01.012.

Backbone

• Vector backbone: Fuw
• Backbone size w/o insert (bp): 9216
• Total vector size (bp): 16359
• Modifications to backbone: Fuw-AscI-EcoRI-HA-P2A-tagBFP
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9-Tet1CD-HA-P2A-BFP
• Species: Synthetic
• Insert Size (bp): 7143
• Mutation: dCas9-D10A, dCas9-H840A
• Promoter: Ubc
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: aactatgcgctcggg
• 3′ sequencing primer: taaagcagcgtatcc

Resource Information

• Supplemental Documents:
  • FUW-dCas9-Tet1CD-HA-P2A-tagBFP with GGGGS.gb
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry