pTRIPZ-EGFP:WTIP
(Plasmid #108231)

• Purpose: Lentiviral vector for Tet-inducible WTIP expression
• Depositing Lab: Kenneth Irvine
• Publication: Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700. doi: 10.1242/jcs.214700.

Backbone

• Vector backbone: pTRIPZ
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 13320
• Total vector size (bp): 14724
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WTIP
• Species: H. sapiens (human)
• Insert Size (bp): 2148
• GenBank ID: NM_001080436.1
• Entrez Gene: WTIP
• Promoter: Tet/ON Minimal CMV promoter
• Tag / Fusion Protein:
  • EGFP

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAACCGTCAGATCGCAATGGTGAGCAAGGGCGAG
• 3′ sequencing primer: CCTCATTCAAACAAACTATTAAAGCAAGTAAAACCTCTACAAATGTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that due to a cloning artifact, the amino acids SLDN were added to the C-terminus of WTIP. The construct will function as described in the associated publication.