pET/LEGH
(Plasmid
#108226)
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PurposeBacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-15b
- Backbone size w/o insert (bp) 5708
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLBT-EGFP-His6
- Promoter T7, lacO
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET/LEGH was a gift from Didier Picard (Addgene plasmid # 108226 ; http://n2t.net/addgene:108226 ; RRID:Addgene_108226) -
For your References section:
Luminescence resonance energy transfer between genetically encoded donor and acceptor for protein-protein interaction studies in the molecular chaperone HSP70/HSP90 complexes. Bhattacharya K, Bernasconi L, Picard D. Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.1038/s41598-018-21210-6. 10.1038/s41598-018-21210-6 PubMed 29434293
