pHCA/GAL4(848).ER
(Plasmid #108215)

• Purpose: Yeast expression vector for fusion of Gal4 AA 1-848 to hormone binding domain of estrogen receptor a
• Depositing Lab: Didier Picard
• Publication: Picard lab plasmids (unpublished)

Backbone

• Vector backbone: pHCA
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gal4-ERalpha HBD
• Mutation: ERalpha HBD has G400V mutation
• Promoter: ADH0

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid published in Picard, D. (1999). In Nuclear receptors: a practical approach; D. Picard, ed., Vol. 207 (Oxford: Oxford University Press), pp. 261-274.