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Addgene

pMP71-HLA-A0201-His
(Plasmid #108214)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108214 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMP71
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6759
  • Modifications to backbone
    converted to become Gateway compatible, see this paper: PMID: 22132171
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    major histocompatibility complex, class I, B
  • Alt name
    HLA-A0201
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1115
  • Entrez Gene
    HLA-A (a.k.a. HLAA)
  • Tag / Fusion Protein
    • his (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP71-HLA-A0201-His was a gift from Sébastien Walchli (Addgene plasmid # 108214 ; http://n2t.net/addgene:108214 ; RRID:Addgene_108214)
  • For your References section:

    Breadth and Dynamics of HLA-A2- and HLA-B7-Restricted CD8(+) T Cell Responses against Nonstructural Viral Proteins in Acute Human Tick-Borne Encephalitis Virus Infection. Lampen MH, Uchtenhagen H, Blom K, Varnaite R, Pakalniene J, Dailidyte L, Walchli S, Lindquist L, Mickiene A, Michaelsson J, Schumacher TN, Ljunggren HG, Sandberg JK, Achour A, Gredmark-Russ S. Immunohorizons. 2018 Jul 2;2(6):172-184. doi: 10.4049/immunohorizons.1800029. 10.4049/immunohorizons.1800029 PubMed 31022685