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Addgene

pS3C
(Plasmid #108211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Merck Millipore
  • Backbone size w/o insert (bp) 5330
  • Total vector size (bp) 7367
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Supplement with 1% glucose is recommended to suppress leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SsoPCNA3-CYP101A1 fusion protein
  • Species
    Synthetic; Pseudomonas putida, Sulfolobus solfataricus P2
  • Insert Size (bp)
    2040
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His6 tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminal
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pS3C was a gift from Teruyuki Nagamune (Addgene plasmid # 108211 ; http://n2t.net/addgene:108211 ; RRID:Addgene_108211)

  • For your References section:

    A Stable Artificial Multienzymatic Complex Using a Heterotrimeric Protein From Metallosphaera sedula. Iwata F, Hirakawa H, Nagamune T. Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30. 10.1002/biot.201700662 PubMed 29663675
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