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Addgene

pBsaGSCYP101A1
(Plasmid #108206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHSG398
  • Backbone manufacturer
    TaKaRa
  • Backbone size w/o insert (bp) 2230
  • Total vector size (bp) 3503
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYP101A1
  • Alt name
    P450cam
  • Species
    Synthetic; Pseudomonas putida
  • Insert Size (bp)
    1300
  • Promoter lac promoter

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBsaGSCYP101A1 was a gift from Teruyuki Nagamune (Addgene plasmid # 108206 ; http://n2t.net/addgene:108206 ; RRID:Addgene_108206)

  • For your References section:

    A Stable Artificial Multienzymatic Complex Using a Heterotrimeric Protein From Metallosphaera sedula. Iwata F, Hirakawa H, Nagamune T. Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30. 10.1002/biot.201700662 PubMed 29663675
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