pS3GG
(Plasmid
#108199)
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PurposeS. solfataricus PCNA3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28b(+)
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Backbone manufacturerMerck Millipore
- Backbone size w/o insert (bp) 5330
- Total vector size (bp) 6121
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsSupplement with 1% glucose is recommended to suppress leaky expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameS. solfataricus PCNA3
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Alt namessoPCNA3
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SpeciesSynthetic; Sulfolobus solfataricus P2
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Insert Size (bp)790
- Promoter T7 promoter
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Tags
/ Fusion Proteins
- His6 tag (N terminal on backbone)
- His6 tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pS3GG was a gift from Teruyuki Nagamune (Addgene plasmid # 108199 ; http://n2t.net/addgene:108199 ; RRID:Addgene_108199) -
For your References section:
A Stable Artificial Multienzymatic Complex Using a Heterotrimeric Protein From Metallosphaera sedula. Iwata F, Hirakawa H, Nagamune T. Biotechnol J. 2018 Nov;13(11):e1700662. doi: 10.1002/biot.201700662. Epub 2018 Apr 30. 10.1002/biot.201700662 PubMed 29663675