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Addgene

pSUPER.PKCdelta.RNAi
(Plasmid #10819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC delta RNAi
  • Alt name
    PKC delta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    PRKCD (a.k.a. ALPS3, CVID9, MAY1, PKCD, nPKC-delta)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGAC AAGATCATCG GCAGATTTCA AGAGAATCTG CCGATGATCT TGTCTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGACAAG ATCATCGGCA GATTCTCTTG AAATCTGCCG ATGATCTTGT CGGG-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER.PKCdelta.RNAi was a gift from Alex Toker (Addgene plasmid # 10819 ; http://n2t.net/addgene:10819 ; RRID:Addgene_10819)

  • For your References section:

    Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Storz P, Doppler H, Toker A. Mol Cell Biol. 2004 Apr . 24(7):2614-26. 10.1128/MCB.24.7.2614-2626.2004 PubMed 15024053
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