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Addgene

pSUPER.PKD3.RNAi
(Plasmid #10818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10818 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKD3 RNAi
  • Alt name
    PKD3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    PRKD3 (a.k.a. EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGTC CTAAGACGGG ACTCTCTTCA AGAGAGAGAG TCCCGTCTTA GGACTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAGTCCTA AGACGGGACT CTCTCTCTTG AAGAGAGTCC CGTCTTAGGA CGGG-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER.PKD3.RNAi was a gift from Alex Toker (Addgene plasmid # 10818 ; http://n2t.net/addgene:10818 ; RRID:Addgene_10818)

  • For your References section:

    Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex. Hausser A, Storz P, Martens S, Link G, Toker A, Pfizenmaier K. Nat Cell Biol. 2005 Sep . 7(9):880-6. 10.1038/ncb1289 PubMed 16100512
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