pSUPER.PKD1.RNAi
(Plasmid #10815)

• Depositing Lab: Alex Toker
• Publication: Storz et al EMBO J. 2003 Jan 2. 22(1):109-20.

Backbone

• Vector backbone: pSUPER
• Backbone size w/o insert (bp): 3176
• Vector type: Mammalian Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKD1 RNAi
• Alt name: PKD1
• Species: H. sapiens (human)
• Insert Size (bp): 64
• Entrez Gene: PRKD1 (a.k.a. CHDED, PKC-MU, PKCM, PKD, PRKCM)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCATG CTGTGGGGGC TGGTACTTCA AGAGAGTACC AGCCCCCACA GCATTTTTTG GAAA-3' and 5'-AGCTTTTCCA AAAAATGCTG TGGGGGCTGG TACTCTCTTG AAGTACCAGC CCCCACAGCA TGGG-3'.