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Addgene

mCherry-FKBP-SAC1-HLx8
(Plasmid #108136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587)
  • Species
    H. sapiens (human)
  • Mutation
    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)
  • Entrez Gene
    SACM1L (a.k.a. SAC1)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The Sac1 insert contains a Y434F mutation compared to the closest reference sequence (NP_054735.3). The depositor has noted that this is a common point mutation that does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-FKBP-SAC1-HLx8 was a gift from Gerry Hammond (Addgene plasmid # 108136 ; http://n2t.net/addgene:108136 ; RRID:Addgene_108136)

  • For your References section:

    SAC1 degrades its lipid substrate PtdIns4P in the endoplasmic reticulum to maintain a steep chemical gradient with donor membranes. Zewe JP, Wills RC, Sangappa S, Goulden BD, Hammond GR. Elife. 2018 Feb 20;7. pii: 35588. doi: 10.7554/eLife.35588. 35588 [pii] PubMed 29461204
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