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PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 108120 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepC1
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Backbone manufacturerEvrogen
- Backbone size w/o insert (bp) 3937
- Total vector size (bp) 5578
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSypHer3s-IMS
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SpeciesSynthetic
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Insert Size (bp)1641
- Promoter CMV
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Tag
/ Fusion Protein
- ratiometricYFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I.М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, upcoming publication
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC1-SypHer3s-IMS was a gift from Vsevolod Belousov (Addgene plasmid # 108120 ; http://n2t.net/addgene:108120 ; RRID:Addgene_108120) -
For your References section:
SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range. Ermakova YG, Pak VV, Bogdanova YA, Kotlobay AA, Yampolsky IV, Shokhina AG, Panova AS, Marygin RA, Staroverov DB, Bilan DS, Sies H, Belousov VV. Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c. 10.1039/c7cc08740c PubMed 29497715
