pC1-SypHer3s-dmito
(Plasmid #108119)

• Purpose: Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.
• Depositing Lab: Vsevolod Belousov
• Publication: Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.1039/c7cc08740c.

Backbone

• Vector backbone: pC1
• Backbone manufacturer: Evrogen
• Backbone size w/o insert (bp): 3931
• Total vector size (bp): 5539
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SypHer3s-dmito
• Alt name: mitochondrial SypHer3s; MLS1-2 SypHer3s
• Species: Synthetic
• Insert Size (bp): 1608
• Promoter: CMV
• Tag / Fusion Protein:
  • ratiometric YFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• Supplemental Documents:
  • pC1-SypHer3s-dmito
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

. Ermakova Y. G., Pak V. V., Bogdanova Y. A., Kotlobay A. A., Yampolsky I. М., Shokhina A. G., A. S. Panova, R. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov “SypHer3s: genetically encoded fluorescent ratiometric probe with enhanced brightness and improved dynamic range”, Chemical Communications, 2017, accepted publication