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LentiV_Neo_LKB1_CR
(Plasmid #108112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LentiV_Neo
  • Backbone size w/o insert (bp) 7210
  • Total vector size (bp) 8557
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LKB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1299
  • Mutation
    change cytosine 333 to alanine (silent mutation)
  • GenBank ID
    NM_000455
  • Entrez Gene
    STK11 (a.k.a. LKB1, PJS, hLKB1)
  • Promoter EFS promoter
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer AACTGGGAAAGTGATGTCGTGTACTG
  • 3′ sequencing primer GAGAACCTGCGTGCAATCCATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiV_Neo_LKB1_CR was a gift from Christopher Vakoc (Addgene plasmid # 108112 ; http://n2t.net/addgene:108112 ; RRID:Addgene_108112)

  • For your References section:

    LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia. Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR. Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011. 10.1016/j.molcel.2018.02.011 PubMed 29526696
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