LentiV_Neo_LKB1
(Plasmid #108111)

• Purpose: Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene
• Depositing Lab: Christopher Vakoc
• Publication: Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.

Backbone

• Vector backbone: LentiV_Neo
• Backbone size w/o insert (bp): 7210
• Total vector size (bp): 8557
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LKB1
• Species: H. sapiens (human)
• Insert Size (bp): 1299
• GenBank ID: NM_000455
• Entrez Gene: STK11 (a.k.a. LKB1, PJS, hLKB1)
• Promoter: EFS promoter
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AACTGGGAAAGTGATGTCGTGTACTG
• 3′ sequencing primer: GAGAACCTGCGTGCAATCCATC

Resource Information

• Supplemental Documents:
  • lentiV_Neo_Flag-LKB1.ape
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry