LentiV_Neo_SIK3_CR_T221E
(Plasmid #108108)

• Purpose: Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene
• Depositing Lab: Christopher Vakoc
• Publication: Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.

Backbone

• Vector backbone: LentiV_Neo
• Backbone size w/o insert (bp): 7210
• Total vector size (bp): 10989
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIK3
• Species: H. sapiens (human)
• Insert Size (bp): 3783
• Mutation: change guanine 501 to cytosine (silent mutation), change Threonine 221 to Glutamic acid
• GenBank ID: NM_001281749
• Entrez Gene: SIK3 (a.k.a. L19, QSK, SIK-3)
• Promoter: EFS promoter

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: AACTGGGAAAGTGATGTCGTGTACTG
• 3′ sequencing primer: GAGAACCTGCGTGCAATCCATC

Resource Information

• Supplemental Documents:
  • lentiV_Neo_hSIK3v3_T221E (G501C).ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry