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Addgene

pBabe(puro) mouse Egr4
(Plasmid #108105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5086
  • Total vector size (bp) 6537
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Egr4
  • Alt name
    Early growth response 4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1437
  • GenBank ID
    NM_020596.2
  • Entrez Gene
    Egr4 (a.k.a. NGF1-C, NGFI-C, NGFIC, pAT133)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBabe 5'
  • 3′ sequencing primer pBabe 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Egr4 gene was transferred from pcDNA3.1-Egr4 (Addgene plasmid 108096) to the pBabe(puro) retroviral vector using EcoRI and SalI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe(puro) mouse Egr4 was a gift from Peter Johnson (Addgene plasmid # 108105 ; http://n2t.net/addgene:108105 ; RRID:Addgene_108105)

  • For your References section:

    An Arf-Egr-C/EBPbeta pathway linked to ras-induced senescence and cancer. Salotti J, Sakchaisri K, Tourtellotte WG, Johnson PF. Mol Cell Biol. 2015 Mar;35(5):866-83. doi: 10.1128/MCB.01489-14. Epub 2014 Dec 22. 10.1128/MCB.01489-14 PubMed 25535333
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