pISH-Vmn2r120-TR
(Plasmid
#108087)
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PurposePreparation of RNA probe for ISH of mouse Vmn2r120 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108087 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
- Total vector size (bp) 3711
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVmn2r120
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SpeciesM. musculus (mouse)
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Insert Size (bp)696
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Mutationchanged A to G at nt 1196 of NM_001104591.1
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Entrez GeneVmn2r120 (a.k.a. EG224916)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pISH-Vmn2r120-TR was a gift from Peter Mombaerts (Addgene plasmid # 108087 ; http://n2t.net/addgene:108087 ; RRID:Addgene_108087) -
For your References section:
Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1. Akiyoshi S, Ishii T, Bai Z, Mombaerts P. Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub 2018 Mar 25. 10.1111/ejn.13875 PubMed 29465786