Vmn2r1-5Hprt-loxP TV
(Plasmid
#108079)
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PurposeTergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108079 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK+
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Backbone manufacturerAgilent Technologies/Stratagene
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 15000
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Vector typeMouse Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1-Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVmn2r1-Hprt (5')-loxP-neo
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Alt nameVmn2r1
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SpeciesM. musculus (mouse)
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Insert Size (bp)12189
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Entrez GeneVmn2r1 (a.k.a. Casrl1, EG56544, V2r83)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Vmn2r1-5Hprt-loxP TV was a gift from Peter Mombaerts (Addgene plasmid # 108079 ; http://n2t.net/addgene:108079 ; RRID:Addgene_108079) -
For your References section:
Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1. Akiyoshi S, Ishii T, Bai Z, Mombaerts P. Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub 2018 Mar 25. 10.1111/ejn.13875 PubMed 29465786