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Addgene

MAC-tag-C
(Plasmid #108077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CCDB
  • Insert Size (bp)
    306
  • Entrez Gene
    ccdB(letD) (a.k.a. D616_p97105, Fpla045)
  • Tag / Fusion Protein
    • StrepIII-HA-BirA* (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAC-tag-C was a gift from Markku Varjosalo (Addgene plasmid # 108077 ; http://n2t.net/addgene:108077 ; RRID:Addgene_108077)

  • For your References section:

    An AP-MS- and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Liu X, Salokas K, Tamene F, Jiu Y, Weldatsadik RG, Ohman T, Varjosalo M. Nat Commun. 2018 Mar 22;9(1):1188. doi: 10.1038/s41467-018-03523-2. 10.1038/s41467-018-03523-2 PubMed 29568061
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