Skip to main content
Addgene

pSUPER.PKCzeta.RNAi
(Plasmid #10803)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10803 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSUPER
  • Backbone size w/o insert (bp) 3176
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC zeta RNAi
  • Alt name
    PKC zeta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    PRKCZ (a.k.a. PKC-ZETA, PKC2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by ligation of the following oligonucleotide pairs to pSUPER: 5'-GATCCCCGAT
GGAGGAAGCT
GTACCGTTCA
AGAGACGGTA
CAGCTTCCTC
CATCTTTTTG
GAAA-3' and 5'-AGCTTTTCCA AAAAGATGGA GGAAGCTGTA CCGTCTCTTG AACGGTACAG CTTCCTCCAT CGGG-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER.PKCzeta.RNAi was a gift from Alex Toker (Addgene plasmid # 10803 ; http://n2t.net/addgene:10803 ; RRID:Addgene_10803)

  • For your References section:

    Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Storz P, Doppler H, Toker A. Mol Cell Biol. 2004 Apr . 24(7):2614-26. 10.1128/MCB.24.7.2614-2626.2004 PubMed 15024053
Related items:
Commonly requested with: