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Addgene

Myr.PKCzeta.FLAG
(Plasmid #10802)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6
  • Backbone size w/o insert (bp) 4650
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC zeta
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Prkcz (a.k.a. 14-3-3-zetaisoform, Pkcz, r14-3-3)
  • Tags / Fusion Proteins
    • Myr (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site see map (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constitutively active. Myr sequence from p60src. Note: There is a HindIII site in the insert ~1100bp from the 5'end.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myr.PKCzeta.FLAG was a gift from Alex Toker (Addgene plasmid # 10802 ; http://n2t.net/addgene:10802 ; RRID:Addgene_10802)

  • For your References section:

    Regulation of protein kinase C zeta by PI 3-kinase and PDK-1. Chou MM, Hou W, Johnson J, Graham LK, Lee MH, Chen CS, Newton AC, Schaffhausen BS, Toker A. Curr Biol. 1998 Sep 24. 8(19):1069-77. 10.1016/S0960-9822(98)70444-0 PubMed 9768361
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