pcDNA 3.1(-) mouse Egr2
(Plasmid #107997)

• Purpose: Expresses mouse Egr2 in mammalian cells
• Depositing Lab: Peter Johnson
• Publication: Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. doi: 10.1128/MCB.01489-14. Epub 2014 Dec 22.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 6854
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Egr2
• Alt name: Early growth response 2
• Species: M. musculus (mouse)
• Insert Size (bp): 1413
• GenBank ID: NM_010118.3
• Entrez Gene: Egr2 (a.k.a. Egr-2, Krox-20, Krox20, NGF1-B, Zfp-25, Zfp-6)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A Sal I site was introduced before the HindIII site to further transfer to pBabe.