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Addgene

pGEX-6P1-OPNc-delta S
(Plasmid #107985)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107985 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPNc 999bp-16aa signal
  • Species
    H. sapiens (human)
  • Mutation
    WT splice variant
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P1-OPNc-delta S was a gift from Georg Weber (Addgene plasmid # 107985 ; http://n2t.net/addgene:107985 ; RRID:Addgene_107985)

  • For your References section:

    An osteopontin splice variant induces anchorage independence in human breast cancer cells. He B, Mirza M, Weber GF. Oncogene. 2006 Apr 6;25(15):2192-202. doi: 10.1038/sj.onc.1209248. 10.1038/sj.onc.1209248 PubMed 16288209
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