pGEX-6P1-OPNb-delta S
(Plasmid
#107984)
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Purposebacterial expression of OPN splice variant b
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107984 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6P1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOPNb 1038bp-17aa signal
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SpeciesH. sapiens (human)
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MutationWT splice variant
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Entrez GeneSPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that Addgene NGS returned several ambiguous bases in the OPNb ORF (1328Y, 1526R, 1532Y, 1610Y), but none of this would alter the amino acid sequence of the protein.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-6P1-OPNb-delta S was a gift from Georg Weber (Addgene plasmid # 107984 ; http://n2t.net/addgene:107984 ; RRID:Addgene_107984) -
For your References section:
An osteopontin splice variant induces anchorage independence in human breast cancer cells. He B, Mirza M, Weber GF. Oncogene. 2006 Apr 6;25(15):2192-202. doi: 10.1038/sj.onc.1209248. 10.1038/sj.onc.1209248 PubMed 16288209
