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Addgene

pCR3.1-OPNb
(Plasmid #107981)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107981 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    osteopontin splice variant b
  • Species
    H. sapiens (human)
  • Mutation
    splice variant
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3.1-OPNb was a gift from Georg Weber (Addgene plasmid # 107981 ; http://n2t.net/addgene:107981 ; RRID:Addgene_107981)

  • For your References section:

    An osteopontin splice variant induces anchorage independence in human breast cancer cells. He B, Mirza M, Weber GF. Oncogene. 2006 Apr 6;25(15):2192-202. doi: 10.1038/sj.onc.1209248. 10.1038/sj.onc.1209248 PubMed 16288209
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