pCR3.1-OPNa
(Plasmid
#107980)
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Purposeexpression of full-length OPN
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 107980 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefull-length osteopontin
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SpeciesH. sapiens (human)
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Entrez GeneSPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR3.1-OPNa was a gift from Georg Weber (Addgene plasmid # 107980 ; http://n2t.net/addgene:107980 ; RRID:Addgene_107980) -
For your References section:
An osteopontin splice variant induces anchorage independence in human breast cancer cells. He B, Mirza M, Weber GF. Oncogene. 2006 Apr 6;25(15):2192-202. doi: 10.1038/sj.onc.1209248. 10.1038/sj.onc.1209248 PubMed 16288209