pCRB DRT7VTPLux*500CFP
(Plasmid
#107960)
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PurposeCFP half-circuit controller plasmid [ca. 558.5 relative RBS strength at PLas81*]
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107960 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepR33S175
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameT7RNAP (modified)
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional genes: mTurquoise2, luxR, lasR . Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRB DRT7VTPLux*500CFP was a gift from Jim Haseloff (Addgene plasmid # 107960 ; http://n2t.net/addgene:107960 ; RRID:Addgene_107960) -
For your References section:
Programmed hierarchical patterning of bacterial populations. Boehm CR, Grant PK, Haseloff J. Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3. 10.1038/s41467-018-03069-3 PubMed 29472537