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Addgene

FLAG.PKCepsilon.K/W
(Plasmid #10796)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKC epsilon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Mutation
    Kinase dead mutant: K437W (Lys 437 to Trp)
  • Entrez Gene
    PRKCE (a.k.a. PKCE, nPKC-epsilon)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG.PKCepsilon.K/W was a gift from Alex Toker (Addgene plasmid # 10796 ; http://n2t.net/addgene:10796 ; RRID:Addgene_10796)

  • For your References section:

    Regulation of novel protein kinase C epsilon by phosphorylation. Cenni V, Doppler H, Sonnenburg ED, Maraldi N, Newton AC, Toker A. Biochem J. 2002 May 1. 363(Pt 3):537-45. 10.1042/0264-6021:3630537 PubMed 11964154
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