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Addgene

pCRB DRT7VTPLux*500
(Plasmid #107959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pR33S175
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    T7RNAP (modified)
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional genes: mVenus, mTurquoise2, luxR, lasR . Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRB DRT7VTPLux*500 was a gift from Jim Haseloff (Addgene plasmid # 107959 ; http://n2t.net/addgene:107959 ; RRID:Addgene_107959)

  • For your References section:

    Programmed hierarchical patterning of bacterial populations. Boehm CR, Grant PK, Haseloff J. Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3. 10.1038/s41467-018-03069-3 PubMed 29472537
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