p4g3R
(Plasmid
#107958)
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PurposeRFP reporter [PT7(-3G)-RBSB0034, low copy number]
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB4A5
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemRFP1
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4g3R was a gift from Jim Haseloff (Addgene plasmid # 107958 ; http://n2t.net/addgene:107958 ; RRID:Addgene_107958) -
For your References section:
Programmed hierarchical patterning of bacterial populations. Boehm CR, Grant PK, Haseloff J. Nat Commun. 2018 Feb 22;9(1):776. doi: 10.1038/s41467-018-03069-3. 10.1038/s41467-018-03069-3 PubMed 29472537