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pCFJ150-GFP(dpiRNA)::CDK-1
(Plasmid #107938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFJ150
  • Total vector size (bp) 10169
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP(dpiRNA)::CDK-1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2065
  • GenBank ID
  • Entrez Gene
    cdk-1 (a.k.a. CELE_T05G5.3)
  • Promoter cdk-1

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTTATAATACGACTCACTAGTGGG
  • 3′ sequencing primer GTACCAGAGCTCACCTAGGTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ150-GFP(dpiRNA)::CDK-1 was a gift from Heng-Chi Lee (Addgene plasmid # 107938 ; http://n2t.net/addgene:107938 ; RRID:Addgene_107938)

  • For your References section:

    The piRNA targeting rules and the resistance to piRNA silencing in endogenous genes. Zhang D, Tu S, Stubna M, Wu WS, Huang WC, Weng Z, Lee HC. Science. 2018 Feb 2;359(6375):587-592. doi: 10.1126/science.aao2840. Epub 2018 Feb 1. 10.1126/science.aao2840 PubMed 29420292
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