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pAAV2-SBECN1W
(Plasmid #107937)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107937 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5880
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mouse Beclin-1 (BECN1)
  • Alt name
    mBECN1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1345
  • GenBank ID
    NM_019584.3
  • Entrez Gene
    Becn1 (a.k.a. Atg6)
  • Promoter hSynapsin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI, NheI (not destroyed)
  • 3′ cloning site SpeI, HindIII (not destroyed)
  • 5′ sequencing primer AGTCGTGTCGTGCCTGAGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV2-SBECN1W was a gift from Johan Jakobsson (Addgene plasmid # 107937 ; http://n2t.net/addgene:107937 ; RRID:Addgene_107937)

  • For your References section:

    Huntingtin Aggregation Impairs Autophagy, Leading to Argonaute-2 Accumulation and Global MicroRNA Dysregulation. Pircs K, Petri R, Madsen S, Brattas PL, Vuono R, Ottosson DR, St-Amour I, Hersbach BA, Matusiak-Bruckner M, Lundh SH, Petersen A, Deglon N, Hebert SS, Parmar M, Barker RA, Jakobsson J. Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 10.1016/j.celrep.2018.07.017. 10.1016/j.celrep.2018.07.017 PubMed 30089251
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