pAAV-hSyn-Htt171-66Q-myc-WPRE
(Plasmid #107934)

• Purpose: Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons
• Depositing Lab: Johan Jakobsson
• Publication: Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. doi: 10.1016/j.celrep.2018.07.017.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 5249
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Exon 1 of mutant HTT, 66 polyQ repeats
• Alt name: mutantHTT
• Alt name: mHTT
• Species: H. sapiens (human)
• Insert Size (bp): 723
• GenBank ID: NM_002111.7
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: hSynapsin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI, NheI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: AGTCGTGTCGTGCCTGAGA

Resource Information

• Supplemental Documents:
  • 1267 pAAV2-SnHtt66Q(915)W.ape
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry