pET-53-RtcA
(Plasmid
#107876)
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PurposeExpresses E. coli RtcA in bacterial expression cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 107876 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-53-DEST
- Total vector size (bp) 6191
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor expression, use strain: BL21 (DE3) RIPL
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE. coli RtcA
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SpeciesEscherichia coli
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Insert Size (bp)1050
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Entrez GenertcA (a.k.a. b4475, ECK3406, b3419, b3420, yhgJ, yhgK)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-53-RtcA was a gift from Jay Hesselberth (Addgene plasmid # 107876 ; http://n2t.net/addgene:107876 ; RRID:Addgene_107876)
