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Addgene

pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV)
(Plasmid #107860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBI
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARDBP mutant
  • Species
    H. sapiens (human)
  • Mutation
    Deletion of CTD in TDP43 splicing reporter, insertion of EcoRV site
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cut with EcoRV (61bp spacer is lost) and use Gibson Cloning to insert CTD variant. Remove EcoRV during Gibson reaction to prevent unwanted aa insertion.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBS1178 (IBB-GFP-mCherry 3E splicer)-(BFP-P2A-TDP43 dCTD EcoRV) was a gift from Rajat Rohatgi (Addgene plasmid # 107860 ; http://n2t.net/addgene:107860 ; RRID:Addgene_107860)
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