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Addgene

pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9
(Plasmid #107857)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107857 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX458
  • Backbone manufacturer
    Feng Zhang (Addgene plasmid # 48138)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA against TARDBP (Exon 1)
  • gRNA/shRNA sequence
    gRNA against TARDBP (Exon 1)
  • Species
    H. sapiens (human)
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9 was a gift from Rajat Rohatgi (Addgene plasmid # 107857 ; http://n2t.net/addgene:107857 ; RRID:Addgene_107857)

  • For your References section:

    Phase separation-deficient TDP43 remains functional in splicing. Schmidt HB, Barreau A, Rohatgi R. Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. 10.1038/s41467-019-12740-2 PubMed 31653829
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