pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9
(Plasmid
#107857)
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PurposeTo knock-out TDP43
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX458
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Backbone manufacturerFeng Zhang (Addgene plasmid # 48138)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegRNA against TARDBP (Exon 1)
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gRNA/shRNA sequencegRNA against TARDBP (Exon 1)
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SpeciesH. sapiens (human)
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9 was a gift from Rajat Rohatgi (Addgene plasmid # 107857 ; http://n2t.net/addgene:107857 ; RRID:Addgene_107857) -
For your References section:
Phase separation-deficient TDP43 remains functional in splicing. Schmidt HB, Barreau A, Rohatgi R. Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. 10.1038/s41467-019-12740-2 PubMed 31653829