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Addgene

pHBS1292 TDP43 RRM-GFP G368W+W385G
(Plasmid #107838)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107838 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2-Dest
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARDBP mutant
  • Species
    H. sapiens (human)
  • Mutation
    G368W and W385G mutations in LLPS reporter
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHBS1292 TDP43 RRM-GFP G368W+W385G was a gift from Rajat Rohatgi (Addgene plasmid # 107838 ; http://n2t.net/addgene:107838 ; RRID:Addgene_107838)

  • For your References section:

    Phase separation-deficient TDP43 remains functional in splicing. Schmidt HB, Barreau A, Rohatgi R. Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2. 10.1038/s41467-019-12740-2 PubMed 31653829
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