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mmu-miR-30a-5p Block-iT design (BLK)
(Plasmid #107789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6.2-GW/EmGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5699
  • Total vector size (bp) 5760
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mmu-miR-30a-5p mature sequence
  • Alt name
    miR-30a
  • Alt name
    miR-30a-5p
  • gRNA/shRNA sequence
    GTTTTGGCCACTGACTGACCTTCCAGTAGGATGTTTACA
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mir30a (a.k.a. Mirn30a, mir-30a, mmu-mir-30a)
  • Promoter CMV
  • Tag / Fusion Protein
    • EmGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mmu-miR-30a-5p mature sequence was cloned following manufacturer’s instructions. It generates an artificial pre-miRNA, which matures to mmu-miR-30a-5p. Plasmid constructed by Marc Beltrà in the Penna lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mmu-miR-30a-5p Block-iT design (BLK) was a gift from Fabio Penna (Addgene plasmid # 107789 ; http://n2t.net/addgene:107789 ; RRID:Addgene_107789)
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