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Addgene

pMP4518
(Plasmid #107778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1-mcs5
  • Backbone size w/o insert (bp) 800
  • Total vector size (bp) 6400
  • Vector type
    Bacterial Expression
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLacZ-EYFP in pBBR1-mcs5
  • Species
    Aequorea victoria (Jellyfish)
  • Insert Size (bp)
    5600
  • Promoter pLacZ
  • Tag / Fusion Protein
    • pLacZ-EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (unknown if destroyed)
  • 3′ cloning site Xba I (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP4518 was a gift from Herman Spaink (Addgene plasmid # 107778 ; http://n2t.net/addgene:107778 ; RRID:Addgene_107778)

  • For your References section:

    Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants. Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP. Mol Plant Microbe Interact. 2000 Nov;13(11):1163-9. doi: 10.1094/MPMI.2000.13.11.1163. 10.1094/MPMI.2000.13.11.1163 PubMed 11059482
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