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Addgene

pMP4657
(Plasmid #107773)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107773 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME6010
  • Backbone size w/o insert (bp) 5600
  • Total vector size (bp) 14200
  • Vector type
    Bacterial Expression
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLacZ-EGFP in pME6010
  • Species
    Aequorea victoria (Jellyfish)
  • Insert Size (bp)
    5600
  • Promoter pLacZ
  • Tag / Fusion Protein
    • pLacZ-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (unknown if destroyed)
  • 3′ cloning site Sac I (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP from Clontech

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP4657 was a gift from Herman Spaink (Addgene plasmid # 107773 ; http://n2t.net/addgene:107773 ; RRID:Addgene_107773)

  • For your References section:

    Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants. Stuurman N, Pacios Bras C, Schlaman HR, Wijfjes AH, Bloemberg G, Spaink HP. Mol Plant Microbe Interact. 2000 Nov;13(11):1163-9. doi: 10.1094/MPMI.2000.13.11.1163. 10.1094/MPMI.2000.13.11.1163 PubMed 11059482
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