pSG5-HA-ERR3 E275A,R316A
(Plasmid #107768)

• Purpose: Expresses a modified receptor with inactivating mutations in key ligand binding residues
• Depositing Lab: Rebecca Riggins
• Publication: ERR3 plasmid (unpublished)

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5516
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Estrogen-related receptor gamma
• Alt name: ERRg
• Species: M. musculus (mouse)
• Insert Size (bp): 1416
• Mutation: E275A,R316A double mutant
• Entrez Gene: Esrrg (a.k.a. ER, ERR3, Errg, NR3, NR3B3, mKIAA0832)
• Promoter: SV40
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • E275,R316 alignment
• A portion of this plasmid was derived from a plasmid made by: The original, wild type pSG5-HA-ERR3 construct was generated by Dr. Michael Stallcup. Original reference: PMID 10428842. Modifications to Backbone: HA tag and modified multiple cloning site introduced. Original reference for pSG5-HA: PMID 10381882

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry