SDHC-pHLuorin-sems
(Plasmid #107767)

• Purpose: labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements
• Depositing Lab: Karin Busch
• Publication: Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.1038/srep46055.

Backbone

• Vector backbone: pSems26b
• Backbone manufacturer: NEB Biosciences
• Backbone size w/o insert (bp): 5052
• Total vector size (bp): 6390
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Subunit C of SDH fused to pHluorin
• Alt name: SDHC-sEcGFP
• Alt name: SDHC
• Species: H. sapiens (human)
• Insert Size (bp): 1338
• Entrez Gene: SDHC (a.k.a. CYB560, CYBL, PGL3, QPS1, SDH3)
• Promoter: CMV
• Tag / Fusion Protein:
  • pHluorin (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: GFP-R

Resource Information

• Supplemental Documents:
  • SDHC-pHluorin-neo-sems.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry