pNO286-3
(Plasmid #107746)

• Purpose: Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)
• Depositing Lab: Jeffrey Tabor
• Publication: Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone size w/o insert (bp): 2550
• Total vector size (bp): 5409
• Modifications to backbone: Medium copy p15A
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: CcaS
• Alt name: mini CcaS #10
• Species: Synthetic; Synechocystis sp. PCC 6803
• Insert Size (bp): 1389
• Mutation: Deleted AA 223 and 512
• Promoter: J23106

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: GCGAGGAAGCGGAATATATCC

Gene/Insert 2

• Gene/Insert name: Ho1
• Species: Synthetic; Synechocystis sp. PCC 6803
• Insert Size (bp): 723
• GenBank ID:
• Promoter: J23108

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: TAGTAAATCACTGCATAATTCGTGTCG

Gene/Insert 3

• Gene/Insert name: PcyA
• Species: Synthetic; Synechocystis sp. PCC 6803
• Insert Size (bp): 747
• Promoter: J23106

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: GGTGTTCAACAGCCTCACCC

Resource Information

• Supplemental Documents:
  • pno286-3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry