pHCas9-Nours
(Plasmid #107733)

• Purpose: Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
• Depositing Lab: Sheng Yang
• Publication: Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.1002/bit.26827.

Backbone

• Vector backbone: pSH47
• Backbone size w/o insert (bp): 6979
• Total vector size (bp): 11119
• Vector type: Yeast Expression, CRISPR
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cas9
• Species: Human Optimized Streptococcus pyogenes Cas9
• Insert Size (bp): 4140
• Mutation: Human optimized
• Promoter: TEF1
• Tags / Fusion Proteins:
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pHCas9-Nours.gb
• A portion of this plasmid was derived from a plasmid made by: Cas9 gene was amplified from plasmid p415-GalL-Cas9-CYC1t (Addgene:#43804)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry